(with Methylene Blue Counterstain)
APPLICATION
Mammalian Tissue
DESIGNED TO SHOW
Red staining of acid fast bacteria
SPECIMEN REQUIREMENTS
Fixation: Tissue: Any well fixed tissue, however, it is suggested to use formalin fixed tissue.
Sections: Cut Paraffin sections at 5 microns.
COMPONENTS AND REAGENTS
1. Fuchsin Stain Solution - 8oz / 240ml
2. Phenol Dilute Solution - 8oz / 240ml
3. Decolorizing Solution - 8oz / 240ml
4. Methylene Blue Solution - 8oz / 240ml
5. Control Slides 1 Stained and 3 unstained
6. Pap Jars
* Working Carbol Fuchsin Stain
Add 1 part Fuchsin Stain Solution to 6 parts Phenol Solution. Mix . Filtration is necessary.
STAINING PROCEDURE
1. Deparaffinize sections and hydrate to deionized water.
2. Stain sections in * Working Carbol Fuchsin Stain for 20 minutes.
3. Wash sections under tap for water for 5 minutes.
4. Differentiate sections in Decolorization Solution until sections appear pale pink.
5. Wash under tap water two minutes.
6. Counterstain in Methylene Blue Solutions for 2 to 3 seconds.
7. Dehydrate through three changes of absolute alcohol.
8. Clear through two changes of Xylene or Xylene Substitute.
RESULTS
Acid Fast Bacteria - Red
Background - Blue
NOTES
1. Working Carbol Fuchsin Stain has a shelf life of 12 months. Some filtration may have to be performed due to air and temperature during shelf life.
REFERENCES
1. AFIP, Manual of Histologic Staining Methods, McGraw Hill Book Co., 1968,
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