- Catalog Order # K7024A - 4oz. kit - $61.00
- K7028A - 8oz. kit - $101.00
APPLICATION
Mammalian Tissue
DESIGNED TO SHOW
Gram Positive and Negative bacteria
SPECIMEN REQUIREMENTS
Fixation: Tissue: Any well fixed tissue, however, it is suggested to use formalin fixed tissue.
Fixation; Smears: Air dried and alcohol fixed.
Sections; Cut Paraffin sections at 6 microns.
COMPONENTS AND REAGENTS
1. Gentian Violet Solution - 4oz / 120ml
2. Iodine Solution - 4oz / 120ml
3. Solution A - 4oz / 120ml
4. Fuchsin Solution - 4oz / 120ml
5. Solution B - 4oz / 120ml
6. Counterstain Metanil Yellow - 4oz / 120ml
7. Control Slides1 Stained and 3 unstained
8. Pap Jars
STAINING PROCEDURE
1. Deparaffinize sections and hydrate to deionized water.
2. Stain sections in Gentian Violet Stain for 30 seconds.
3. Wash sections in running tap water until excess stain is removed.
4. Treat sections in Iodine Solution for 1 minute.
5. Rinse sections thoroughly under tap water.
6. Treat sections in Solution A to remove stain until background is translucent.
7. Rinse sections quickly under tap water.
8. Stain sections in Fuchsin Solution for 2 minutes.
9. Wash sections quickly under running tap or deionized water.
10. Immerse sections in Solution B for 1 minute. Agitate slides during the last 15 seconds.
11. Rinse sections thoroughly under tap or deionized water.
12. Stain sections in Counterstain for 2 minutes. (This step is unnecessary for staining smears)
13. Rinse sections quickly in deionized water and air dry.
14. Clear through two changes of Xylene or Xylene Substitute. (Increase time in Xylene Substitutes)
RESULTS
Gram Positive Cocci-Black/Blue
Negative Bacilli-Red
NucleiRed
BackgroundYellow
NOTES
1. Use agitation while decolorizing in Step 6.
REFERENCES
1. AFIP, Manual of Histologic Staining Methods, American Registry of Pathology, 1992
2. Luna LG: Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts, Gaitherburg, MD, American Histolabs, Inc., 1992
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