- Catalog order # K7124 - 4oz kit - $56.00
- K7128 - 8oz kit - $97.00
APPLICATION
Mammalian Tissue
DESIGNED TO SHOW
Blood, Parasites, H-Pylori, and Nuclear Stain
SPECIMEN REQUIREMENTS
Fixation: Tissue: Any well fixed tissue , however, it is suggested to use buffered formalin fixed tissue.
Smears: Allow to air dry before fixing in Solution A .
Sections: Cut Paraffin sections 5 microns.
COMPONENTS AND REAGENTS
1. Solution A - 4 oz / 120ml
2. Solution B - 4 oz / 120 ml
3. Solution C - 4 oz / 120 ml
4. 1 Stained and 3 unstained control slides
STAINING PROCEDURE
1. Deparaffinize sections and hydrate to deionized water.
2. Treat frozen sections and blood smears in Solution A.
3. Dip slides 25 times in Solution B for optimum results.
4. Dip slides 25 times in Solution C.
(Do not rinse slides before treating in Solution C)
5. Rinse quickly under distilled water.
6. Quickly check slides microscopically.
Repeat steps 3-4 if slides need more enhancement.
7. Air dry slides.
8. Clear in Xylene and mount using synthetic mounting medium.
RESULTS
Chromatin of white blood cellPurple to blue
Nuclei of Parasitic protozoaRed to bright pink
Eosinophilic GranulesBright Pink
Neutrophilic GranulesPurple
Red Blood CellsGray
Helicobacter PyloriBlue
NucleiBlue-Purple
CytoplasmPink-Orange
NOTES
1. Deionized water is recommended to be used on all procedure steps. Pending on treated water souce, tap water may vary on quality for staining purposes.
2. Change to fresh stains when intensity of stain decreases.
3. If stain is too intense, differentiate slide in two changes of (not included with kit), 0.25% Acetic Acid. Combine 1 ml of Acetic Acid, Glacial to every 400 ml of distilled water. Following treatment in 0.25% Acetic Acid, rinse slide or smear in deionized water before microscopic examination .
REFERENCES
1. Laboratory Medicine; Vol. 25, No. 6; June 1994; pp. 389
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