- Catalog order # K7144 - 4oz kit - $63.00
- K7148 - 8oz kit - $101.00
APPLICATION
Mammalian Tissue
DESIGNED TO SHOW
Argentaffin Granules and Pigments
SPECIMEN REQUIREMENTS
Fixation: Tissue: Any well fixed tissue , however, it is suggested to use 10% buffered formalin fixed tissue.
Sections: Cut Paraffin sections at 5 microns.
COMPONENTS AND REAGENTS
1. Stabilized Silver Nitrate Solution - 4 oz / 120 ml
2. Gold Chloride Solution - 4 oz / 120 ml
3. Sodium Thiosulfate - 4 oz / 120 ml
4. Nuclear Fast Red - 4 oz / 120 ml
5. 1 Stained and 3 unstained control slides
6. Pap Jars
* Silver Nitrate Working
Add Ammonium Hydroxide (Concentrated) to 20 ml of Stabilized Silver Nitrate Solution until precipitated formed disappears
Add Stabilized Silver Nitrate until solution becomes slightly opalescent. Bring total volume to 100ml using distilled or ultrapure water.
STAINING PROCEDURE
Preheat waterbath or oven to 37 C.
1. Deparaffinize sections and hydrate to deionized water.
2. Place sections in *Silver Nitrate Working and treat in
Preheated waterbath for 30 minutes or until sections are light brown.
Increase time to 60 minutes if heating device is oven.
3. Rinse in three changes of distilled water.
4. Tone sections in Gold Chloride Solution for 10 minutes.
5. Rinse sections in one change of distilled water.
6. Place sections in Sodium Thiosulfate solution for 5 minutes.
7. Rinse sections in one change of distilled water.
8. Counterstain with Nuclear Fast Red for 5 minutes.
9. Rinse thoroughly under deionized or distilled water.
10. Dehydrate sections through one change of 95% Alcohol and three absolute alcohols.
11. Clear sections through Xylene or Xylene substitute. (Xylene substitutes require increase time schedules)
12. Mount sections with Clearslip.
RESULTS
Argentaffin Granules-Black
Melanin-Black
Nuclei and Cytoplasm-Pink to Red
NOTES
1. Procedure is non-specific for melanin.
REFERENCES
1. Masson P. Carcinoids and nerve hyperplasia of the appendicular mucosa. Am J Pathol. ; 1928; 4:181
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