- Catalog order # K7204 - 4oz kit - $71.00
- K7208 - 8oz kit - $111.00
APPLICATION
Mammalian Tissue
DESIGNED TO SHOW
Collagenous fibrils, Cartilage, Mucin, Amyloid, and Elastic fibrils
SPECIMEN REQUIREMENTS
Fixation: Zenkers fixative with Mercuric Chloride.
Sections: Cut Paraffin sections at 5 microns.
COMPONENTS AND REAGENTS
1. Acid Fuchsin Stain - 4 oz / 120 ml
2. AnilineBlue/Orange G Stain Solution - 4 oz / 120 ml
3. Phosphomolybdic Acid Solution - 4 oz / 120 ml
4. One stained and three unstained slides
5. Pap Jars
*Weigerts Iodine Solution (Available from IMEB) S7035
5% Sodium Thiosulate
Add 5 gm of Sodium Thiosulfate to 100 ml of distilled water.
STAINING PROCEDURE
1. Deparaffinize sections and hydrate to deionized water.
2. Treat sections in *Weigerts Iodine Solution for 5 minutes to remove mercury pigments.
3. Treat sections in 5% Sodium Thiosulfate for 3 minutes to remove Iodine.
4. Stain section in Acid Fuchsin Solution for 5 minutes.
5. Mordant section in Phosphotungstic Acid for 15 minutes.
6. Rinse sections in deionized or distilled water.
7. Stain section in Aniline Blue/ Orange G for 60 minutes.
8. Dehydrate section through alcohols.
9. Mount slide with Clearslip.
RESULTS
Nuclei Red
Collagenous Fibrils Blue
Erythrocytes Yellow
Elastic Fibers Pale pink to pale yellow
NOTES
REFERENCES
1. Gomori, G (1936): Am J. Path.; 12:pp. 655-663
2. Histopathologic Technic and Practical Histochemistry; Lillie; Third Edition, McGraw Hill; 1965: pp 457-458
Home Page